Thp1 dual cells were derived from the human thp 1 monocyte cell line by stable integration of two inducible reporter constructs. As a dsrna helicase enzyme rig i is encoded by the ddx58 gene. The cell line was established by transfection using a pta nfkb luciferase reporter vector which contains 4 repeats of nfkb binding sites a minimal promoter upstream of the firefly luciferase coding region along with hygromycin expression vector followed by hygromycin selection. 
				
			
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